I never investigated how or why there were different pitch frequencies within a Tuners parameters. Thanks for the links Jay - and thanks for bringing it up cvillavicencio.
There is a difference, adds more grit and bottom end to what is considered Standard Tuning. Nice option to try if someone is unable to tweak their amp to get a little more 'oomph out of their sound.
I often tune to standard D#, D or even C#. It's what makes the bass purr like a kitten. Many metal bands tune lower. It gives the composition balls. I remember covering God Save the Queen by Motörhead. That tuning was weird. It was right in the middle of D and D#. Let's say D+
Not a bad idea to do, I like keeping a sound that is gritty/dirt, punch, and pretty strong to solid low end - all rolled up in a “smack U in ‘da face” kinda of a feel. I like the sound that I can produce now, but I think I’ll explore this avenue of minor de-tuning and see if it sticks with me or not.